Goat Anti-Rat IgG (H&L), FITC conjugated | Gentaur

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451-AS10 1136-GEN
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Goat Anti-Rat IgG (H&L), FITC conjugated | 451-AS10 1136

Species Reactivity Rat

Host/Isotype Goat / Ig

Class Polyclonal

Type Secondary Antibody

Conjugate TRITCSee Additional Formats

Excitation/Emission Profile View spectra

Form Liquid

Concentration 1 mg/mL

Purification Affinity chromatography

Storage buffer PBS, pH 7.2

Contains 0.09% sodium azide

Storage conditions 4° C, store in dark, DO NOT FREEZE!

RRID AB_469694

Target IgG

Antibody Form Whole Antibody

Description: This goat anti-rat IgG polyclonal antibody reacts with rat IgG and is useful for indirect immunofluorescent staining for flow cytometric analysis.

Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: This polyclonal antibody has been tested by flow cytometric detection of rat primary antibodies. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence).

They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

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Additional Information

100 µL
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