Affinity Purified Horse Anti-HBsAg | 503-1806
Target HBSAg (HBsAg)
Reactivity Hepatitis B Virus (HBV)
Compatible Secondaries Clonality
This HBSAg antibody is un-conjugated Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Purity > 95 % pure
Immunogen HBsAg antibody was raised in rabbit using subtypes ad and ay of human HBsAg as the immunogen.
Concentration Lot specific
Buffer Protein A purified IgG fraction in 10 mM PBS, pH 7.2 with 0.1 % NaN3.
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Hepatitis B surface antigen (HBsAg), was purified from plasma by affinity chromatography on matrix-bound sulphated carbohydrates such as heparin or dextran sulphate. Further purification by precipitation with polyethylene glycol (PEG) 6000 resulted in a highly purified HBsAg preparation.
The overall recovery amounted to about 70% of the total antigen content of the starting plasma. Electron microscopic data revealed mainly 22 nm spherical particles accompanied by few or no filaments. The process is simple, rapid and lends itself readily to large-scale applications.